The draw takes place live on talkSPORT 2 on Tuesday 22 November - moving from the usual Monday slot to avoid a clash with England v Iran in the World Cup.
The draw gets underway from 3.15pm.
Third Round winners bag £4,500 with the losers pocketing £1,250.
National League sides joining the competition will hope to start their path to Wembley to emulate Bromley's win last season.
The FA Trophy final takes place on Sunday 21 May 2023.
To listen to the draw on Tuesday click here.