The match is set to take place on Sunday 2nd August at 3pm, and is the ninth consecutive Promotion Final to be held at the national stadium.
Due to the current Government sporting guidelines during the COVID-19 pandemic, the Final will be behind closed doors.
BT Sport will broadcast each of the five play-off games in the Vanarama National League.
Chief Executive Michael Tattersall said: “The Vanarama National League Promotion Final is the most highly anticipated dates in our calendar.
“We are immensely proud to be staging our showpiece Promotion Final at the world’s most iconic football stadium once again.
“I would like to thank Wembley Stadium and the Premier League for their support in making this happen”.
Details of the schedule for the play-offs across the three National League divisions will be released at the earliest opportunity.