York City and Chester will battle it out for a televised second round tie at home to 2013 winners Wigan Athletic in a match sure to be worth a six-figure sum.
Scarborough smell blood with Forest Green Rovers struggling for form in League Two. Having gone so close to knocking them out in the first round tie earlier this month, can the Seadogs complete the job in Nailsworth?
Slough Town were so proud of their performance against Grimsby in the first game and Scott Davies has called for more bravery as the Rebels board the coach to Blundell Park looking to set up a meeting with Oxford United.
There’s a huge National League North programme going on too and all the title chasers are in action.
New leaders Scunthrope United don’t fancy being deposed days after reclaiming top spot - they made the tricky long trip to King’s Lynn Town.
Tamworth lost the leadership after their poor run continued on Saturday but will be determined to get back on track at struggling Bishop’s Stortford.
Two contenders collide as South Shields head to Spennymoor in a match that means so much to many in the North East.
Alfrerton want to show defeat against Scarborough was a blip as they challenge Rushall Olympic as Boston United go to Peterborough Sports as improving Darlington take on Chorley.
In the National League South, leaders Yeovil Town are looking for a 14th win on the spin in all competitions as Farnborough visit Huish Park.
There’s a Kent derby as Maidstone United look to recover from their roasting at Hampton by adding to Dover Athletic’s woes.
Welling United will move out of the bottom four if they leave Braintree Town with three points. Worthing will reclaim a play-off place if they defeat Dartford.
For all of Tuesday’s fixtures, click here.