In Step 1, Forest Green Rovers and Sutton United join following their relegation from the EFL last season.
They will be joined by Tamworth, Boston United, Yeovil Town and Braintree Town following their promotions from the National League North and South respectively.
Relegated Kidderminster Harriers and Oxford City drop into the National League North while Boreham Wood and Dorking Wanderers are allocated into the National League South.
Eight new sides join the competition this season from Step 3.
Marine, Leamington, Needham Market and Radcliffe will compete in the National League North.
Chesham United, Hornchurch, Enfield Town and Salisbury will compete in the National League South.
Full National League constitution as follows:
National League (Step 1)
AFC Fylde
Aldershot Town
Altrincham
Barnet
Boston United
Braintree Town
Dagenham & Redbridge
Eastleigh
Ebbsfleet United
FC Halifax Town
Forest Green Rovers
Gateshead
Hartlepool United
Maidenhead United
Oldham Athletic
Rochdale
Solihull Moors
Southend United
Sutton United
Tamworth
Wealdstone
Woking
Yeovil Town
York City
National League North (Step 2)
Alfreton Town
Brackley Town
Buxton
Chester
Chorley
Curzon Ashton
Darlington
Farsley Celtic
Hereford
Kidderminster Harriers
King's Lynn Town
Leamington
Marine
Needham Market
Oxford City
Peterborough Sports
Radcliffe
Rushall Olympic
Scarborough Athletic
Scunthorpe United
South Shields
Southport
Spennymoor Town
Warrington Town
National League South (Step 2)
Aveley
Bath City
Boreham Wood
Chelmsford City
Chesham United
Chippenham Town
Dorking Wanderers
Eastbourne Borough
Enfield Town
Farnborough
Hampton & Richmond Borough
Hemel Hempstead Town
Hornchurch
Maidstone United
Salisbury
Slough Town
St Albans City
Tonbridge Angels
Torquay United
Truro City
Welling United
Weston-super-Mare
Weymouth
Worthing