The Arch may be quite far in the distance, but the North and South teams starting out on Saturday will be aware clubs from this level and below have a rich history of going all the way.
Just ask Brackley Town, the 2018 winners, who will need to be fully focused at Hashtag United.
Hornchurch were a Isthmian League Premier Division club when they lifted the trophy at the home of football three years ago and they start another attempt with a trip to Enfield Town.
Russ Penn takes new club Halesowen - and more than 1,000 supporters - to his former club Kidderminster Harriers, and he is sure of a warm reception.
There's a big greater Manchester match-up as Curzon Ashton play host to Macclesfield.
There's a number of same-division clashes and former winners Darlington will meet Buxton, Southport are Warrington Town goback into battle, with Weston-super-Mare playing Weymouth and Dorking Wanderers facing Eastbourne Borough.
Torquay United come up against Truro City with both teams gunning for National League South promotion.
After embarrassing Boston United in the FA Cup, Gainsborough Trinity now want to turn over Chester.
Alfreton Town face Needham Market with Reds chairman Wayne Bradley in risky mood.
He is vowing to buy everyone a drink in the bar if in-form Liam Waldock scores again! Read the full story here.
Boreham Wood have high hopes of a Wembley return and will be happy with a home tie against Felixstowe & Walton United.
Worthing go to Chichester City next month with Spennymoor Town meeting South Shields in a big all North East encounter.
Winning teams will receive £3,750 in prize money with losing teams earning £1,000.
This weekend’s ties:
1 Southport v Warrington Town
2 Scunthorpe United v Warrington Rylands
3 Gainsborough Trinity v Chester
4 Bamber Bridge v Farsley Celtic
5 Spennymoor Town v South Shields
6 Darlington v Buxton
7 Stockton Town v Scarborough Athletic
8 Curzon Ashton v Macclesfield
9 Chorley v Marine
10 Radcliffe v Worksop Town
11 Coleshill Town v Biggleswade Town
12 Alfreton Town v Needham Market
13 Aveley v Hemel Hempstead Town
14 Hashtag United v Brackley Town
15 Oxford City v Sporting Khalsa
16 Enfield Town v Hornchurch
17 Alvechurch v Chasetown
18 Leamington v King’s Lynn Town
19 Hereford v Brentwood Town
20 Royston Town v Rushall Olympic
21 Kidderminster Harriers v Halesowen Town
22 Boreham Wood v Felixstowe & Walton United
23 Peterborough Sports v Chelmsford City
24 Hadley v AFC Telford United
25 St Albans City v Basford United
26 Lewes v AFC Totton
27 Salisbury v Farnborough
28 Yate Town v Hampton & Richmond Borough
29 Folkestone Invicta v Hanwell Town
30 Horsham v Maidstone United
31 Gosport Borough v Tonbridge Angels
32 Welling United v Havant & Waterlooville
33 Slough Town v Chatham Town
34 Chesham United v Chippenham Town
35 Chichester City v Worthing
36 Winchester City v Sittingbourne
37 Torquay United v Truro City
38 Bath City v Chertsey Town
39 Weston Super Mare v Weymouth
40 Dorking Wanderers v Eastbourne Borough