Tie of the round from the guaranteed fixtures- set to take place the weekend of December 4th- sees Vanarama National League high-flyers Chesterfield face a trip to Salford City
The Spireites progressed to face the League Two side having beaten Southend United at the weekend.
Underdogs of Round One, Ian Allinson's St Albans City, face a short trip to fifth-tier league leaders Boreham Wood, who he previously managed until 2015.
Kidderminster Harriers' reward for defeating Grimsby Town is hosting free-scoring FC Halifax Town at Aggborough.
Yeovil Town will definitely face an EFL side after reaching the Second Round, but a replay will decide whether Milton Keynes Dons or Stevenage head to Huish Park.
League One giants Charlton Athletic will be watching on as Altrincham and Gateshead battle it out for the opportunity to face them next month.
Elsewhere, should Stockport County negotiate their way past Bolton Wanderers, a trip to Rotherham United will be on offer for Dave Challinor's men.
Neal Ardley's Solihull Moors could head to Essex with a replay victory over Wigan Athletic, while Rochdale stand in the way of Notts County facing either Sheffield Wednesday or Plymouth Argyle.
FA Cup draw involving National League clubs:
Yeovil Town vs Milton Keynes Dons/Stevenage
Colchester United vs Wigan Athletic or Solihull Moors
Rotherham United vs Bolton Wanderers or Stockport County
Gateshead or Altrincham vs Charlton Athletic
Boreham Wood vs St Albans City
Kidderminster Harriers vs FC Halifax Town
Salford City vs Chesterfield
Rochdale or Notts County vs Sheffield Wednesday or Plymouth Argyle