The National League North leaders host the second-placed Blues looking for a quick bounce back after defeat midweek at Kidderminster Harriers.
Chester have won back-to-back games and have a huge following heading to Lincolnshire as the top two in the division look to break records once again.
Last season 7,511 fans packed into the Attis Arena to break the then-National League North attendance record, broken only by the Iron's play-off clash with Boston later that season.
A win for Scunthorpe could see them five points clear at the summit, with Calum McIntyre's side knowing three points for them could see them regain top spot.
One side hoping neither side win will be Brackley Town.
The Saints were on fire until their midweek setback at Buxton but Gavin Cowan has been adamant his side are still in the title race and they will be if they can come away from Radcliffe with three points.
Kidderminster Harriers' midweek win against Scunthorpe means they are also still in the hunt. It's strugglers Needham Market next for them on Saturday.
No National League North side can boast a better record than Marine over the last eight games.
The Mariners have dropped just two points since mid-February but host a Peterborough Sports side who have just one defeat to their name in their last eight games.
Oxford City ended their eight-game winless streak on Tuesday - another win over Warrington Town might have the Hoops fans confident of survival.
Spennymoor have some business at the other end to tend to.
Graeme Lee's side have lost just once at Brewery Field since the start of November and know a win over basement boys Farsley Celtic might see them take another giant step towards their play-off dream.
Hereford and Curzon meet who both also have play-off dreams while Chorley - who have lost their last two - host Alfreton Town at Victory Park.
Could King's Lynn gatecrash the top seven this weekend? They'll need a win at Buxton who are three without defeat.
Darlington's feint play-off hopes hang in the balance much like Rushall Olympic's chances of survival - a win for either side would be huge.
Leamington and South Shields are both out of form and meet each other while a mid-table clash sees Scarborough Athletic head to Southport looking for a third straight win.
National League North fixtures:
Buxton v King's Lynn Town
Chorley v Alfreton Town
Darlington v Rushall Olympic
Hereford v Curzon Ashton
Kidderminster Harriers v Needham Market
Leamington v South Shields
Marine v Peterborough Sports
Oxford City v Warrington Town
Radcliffe v Brackley Town
Scunthorpe United v Chester
Southport v Scarborough Athletic
Spennymoor Town v Farsley Celtic