Jenner has released a statement explaining why there will be a change at the top at the Tigers.
Gloucester escaped relegation after a great late run - form which saw manager Mike Cook win the National League Manager of the Month, and winger Fabien Robert take the Player award.
But Jenner says the time has arrived where he needs to take a step back.
“It is with much sadness that today I announce my resignation as chairman of Gloucester City,” he said.
“I came to the role, reluctantly, under very difficult circumstances and perhaps during one of the most critical points in the club’s history.
“During a massive period of upheaval and change for the club, both myself and now former Vice Chairman Chris Gage, provided a stability and leadership which saw us retain National League status and with the team we built around us, provided resilience across multiple club roles and delivered progress on the Stadium.
“I spoke some months back to Eamonn about my intentions to step aside at the end of the season, with my family and my rapidly expanding business now demanding more of my time; and we had agreed that this announcement would come once both planning was secured and the funding application submitted.
“I would like to say to Mike Cook, the coaching staff and players, a huge thank you for the work they did both on and off the pitch in securing National League South status for next year. I would also like to say a massive thank you to all of the volunteers at the Club whom I’ve worked very closely with these past two years and whose support, advice and dedication I will be forever grateful for.
“Gloucester City is an incredible club, full of amazing supporters and volunteers, whose history I’m proud to be a part of and whose future is exceedingly bright.
“I’ve no doubt that the club will, with the financial backing it now has at board level and the new Supporters Trust Board now working in tandem with the club, return home to Meadow Park in the very near future.”