Operation Promotion was launched by the club's supporters trust in June to provide cash to bring new players into Blundell Park for the 2015-2016 season.
The Mariners narrowly missed out promotion to the Football League in May and The Mariners Trust had initially hoped to raise £20,000.
Delighted officials say more than 3,500 individual donations had been received from supporters, pictured.
Spokesman Dave Roberts said: "It is an incredible achievement and we are very grateful to every supporter for all of their donations over the past eight weeks.
"It goes to prove Grimsby Town supporters really are the best around and we can't wait to get the season under way."