Clubs from the Vanarama National League North and South are involved for the first time in the competition this season.
Fixtures are scheduled to take place the weekend of September 21/22, with any necessary replays the following midweek.
Ties involving National League North/South Clubs:
Southport v Scarborough Athletic or Marske United
Chester v Altrincham
Curzon Ashton v Blyth Spartans
Bradford (Park Avenue) v Morpeth Town
Irlam v York City
Trafford v Darlington
Gateshead v Ramsbottom United
Guiseley v Stockton Town
Lancaster City or Northwich Victoria v Spennymoor Town
Runcorn Town v Farsley Celtic
Alfreton Town v King’s Lynn Town
Stamford v Boston United
Leamington v Chasetown
Kettering Town v Sutton Coldfield Town or Redditch United
AFC Telford United v Nantwich Town
Kidderminster Harriers v Stafford Rangers
Beaconsfield Town v Hemel Hempstead Town
Corinthian Casuals v Chelmsford City
Tonbridge Angels v Eastbourne Borough
Margate v Concord Rangers
Maidstone United v Cheshunt
Dulwich Hamlet v Bognor Regis Town
Sutton Athletic or Flackwell Heath v Slough Town
Billericay Town v Basildon United
Tooting & Mitcham United v Dorking Wanderers
Enfield Town v Braintree Town
Metropolitan Police or Horsham v Dartford
St Albans City v Hastings United or Worthing
Farnborough v Wealdstone
Welling United v Chipstead
Chesham United v Hampton & Richmond Borough
Sholing v Weymouth
Hereford v Truro City
Havant & Waterlooville v Taunton Town
Cirencester Town v Chippenham Town
Poole Town v Hungerford Town
Swindon Supermarine v Bath City
Oxford City v North Leigh
Kidlington v Gloucester City
Brackley Town v Cinderford Town