League One and League Two teams enter the competition and following Saturday’s excitement, things are about to get serious.
Winners bank a cheque for £41,000, with the matches taking place on the first weekend of November.
It’s all eyes on the Beveree tomorrow with the winners of the Torquay United and Hampton & RB replay welcoming giants Derby County - and possibly the TV cameras.
Hereford, who stunned Bromley last weekend, pictured, have a fantastic tie at home again 2008 winners Portsmouth to look forward to.
Ebbsfleet United are unbeaten - they welcome FC Halifax Town, while Weymouth will fancy their chances of an upset against struggling AFC Wimbledon.
Solihull Moors face Hartlepool United, Woking look to stun Oxford United while National League North club Curzon Ashton have a great game at home to Cambridge United.
Chippenham Town will host Lincoln City if they see off Hendon in Tuesday’s replay.
York City are at Shrewsbury Town while it's Doncaster Rovers for National League North leaders King’s Lynn Town.
There are so all-National League games too - Boreham Wood welcome Eastleigh as Maidenhead United and Dagenham & Redbridge lock horns.
The first round draw in full:
South Shields vs Forest Green Rovers
Bolton Wanderers vs Barnsley
Boreham Wood vs Eastleigh
Curzon Ashton vs Cambridge United
Maidenhead United vs Dagenham & Redbridge
Crawley Town vs Accrington Stanley
Solihull Moors vs Hartlepool United
AFC Fylde vs Gillingham
Peterborough United vs Salford City
Sutton United vs Farnborough
Grimsby Town vs Plymouth Argyle
MK Dons vs Taunton
Ebbsfleet United vs FC Halifax Town
Carlisle United vs Tranmere Rovers
Bracknell Town vs Ipswich Town
Chippenham vs Lincoln City
Sheffield Wednesday vs Morecambe
Hereford vs Portsmouth
Shrewsbury Town vs York City
Buxton vs Merthyr Town
Charlton Athletic vs Coalville Town
Weymouth vs AFC Wimbledon
Newport County vs Colchester United
Stockport County vs Swindon Town
Doncaster Rovers vs King’s Lynn Town
Gateshead vs Stevenage
Fleetwood Town vs Oxford City
Burton Albion vs Needham Market
Bradford City vs Harrogate Town
Port Vale vs Exeter City
Torquay vs Derby County
Bristol Rovers vs Rochdale
Wycombe vs Walsall
Wrexham vs Oldham
Crewe vs Leyton Orient
Barnet vs Chelmsford City
Woking vs Oxford United
Chesterfield vs Northampton Town
Cheltenham Town vs Alvechurch