With the National League top six all out, it's as open as ever with the prestigious silverware up for grabs.
After Forest Green lost to Oxford City, the National League North side's reward is a home game with three-time winners Woking.
There's a huge derby game as Rochdale host Altrincham.
Steve Morison is an FA Trophy legend and his Sutton United side welcome Spennymoor Town.
Aldershot Town have another lower league draw but it won't be easy at Sittingbourne, who sent Southend United packing.
The quarter final matches will take place on Saturday 1 March with winning clubs netting £7,500 and losing clubs taking home £2,000.
Isuzu FA Trophy draw:
Oxford City v Woking
Sutton United v Spennymoor Town
Sittingbourne v Aldershot Town
Rochdale v Altrincham