The Blues head to National League North Kidderminster Harriers with manager Marcus Bignot taking charge of just his fourth game.
Chester haven't had a successful recent history in the competition having failed to get beyond the fourth qualifying round on four of the last five occasions.
Sitting inside of the relegation zone, an FA Cup run could be the start of a turn in fortunes.
"For all clubs in non-league the importance of the FA Cup is clear," said Bignot.
"The reality is we're not going to win the FA Cup but you can be a winner in so many different ways in this competition. The exposure the competition gives you and the financial reward it has is important for a club like ourselves.
"I know a lot about Kidderminster. I've worked closely with Colin Gordon setting up an academy for them in the Midlands. I've watched them quite a few times with my links.
"It's the first time I'm going back and managing rather than watching so that'll be nice.
"Kidderminster are a footballing team too so hopefully it will only add to the spectacle that cup football brings."
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