The Blues - relegated at the weekend from the National League - had already informed the former Solihull manager that his contract won’t be renewed next season.
But even victory over Bromley last night wasn’t enough for him to stay on until the end of the season.
The club tonight released a statement announcing a change would be made immediately.
It read: “Chester Football Club can confirm that they have parted company with First Team Manager, Marcus Bignot, with immediate effect.
“After what has proved to be a difficult season both on and off the pitch, which resulted in the club’s relegation from the Vanarama National League last weekend, the club have decided to end the manager’s contract to facilitate the search for a new Manager. Calum McIntyre has been put in caretaker charge of the team for the remainder of the season.”
City Fans United Chair, Dave Harrington-Wright said: “We know that this has been the toughest season for Chester FC fans to endure since the reformation of the club in 2010, and following the board’s decision last week to not renew Marcus’s contract beyond the end of the season, we see little value in him remaining in the position.
“On behalf of the board of directors, I thank him for all his efforts and wish him the very best in the future.”
On the caretaker appointment of Calum McIntyre, Harrington-Wright said, “Calum has displayed tremendous qualities and has enjoyed great success leading the Chester FC Youth Academy team, on a consistent basis, and I hope everyone will get behind him during these difficult last few weeks of the season.
“Calum has already been carrying out coaching duties with the First Team and we are sure that this will present an opportunity for his continued development at the Club.”