But with Guiseley's success comes misery for their West Yorkshire rivals Bradford (Park Avenue).
Aaron Martin's superb hat-trick put the Avenue to the sword as they slumped to their second 5-0 defeat in four days.
Chester recovered from Danny Livesey's early own goal with a last-gasp leveller from Gary Roberts against Altrincham.
King's Lynn Town triumphed in the clash of the newly-promoted sides.
Goals from Adam Marriott and Sonny Carey got the Linnets on the board against Kettering Town.
It was 500 up for Leamington boss Paul Holleran and his side put on a great display to defeat Brackley Town.
Jack Lane and Jordan Murphy notched to maintain their unbeaten start.
No goals between Southport and Curzon Ashton while Farsley Celtic also held York City to a credible draw at Bootham Crescent.
Gloucester City saw two dismissed as they slipped to defeat to Hereford.
Jake Jackson and Fabien Robert saw red in either half as Keiran Thomas and Tom Owen-Evans bagged for the Bulls.
AFC Telford are on the board after overcoming Kidderminster Harriers at New Bucks Head.
Amari Morgan-Smith's penalty opened the scoring for Alfreton Town but Jake Wright's second-half leveller meant the Reds and Boston shared the spoils.
Tuesday's National League North final scores:
AFC Telford United 2-0 Kidderminster Harriers
Alfreton Town 1-1 Boston United
Bradford (Park Avenue) 0-5 Guiseley
Chester 1-1 Altrincham
Gloucester City 0-2 Hereford
King's Lynn Town 2-1 Kettering
Leamington 2-0 Brackley Town
Southport 0-0 Curzon Ashton
York City 0-0 Farsley Celtic